a32723 rrid ab 2633275 Search Results


a 32723 rrid ab 2633275 critical  (ATCC)
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ATCC a 32723 rrid ab 2633275 critical
A 32723 Rrid Ab 2633275 Critical, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a32723 rrid ab 2633275 goat anti igg h l  (Miltenyi Biotec)
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Miltenyi Biotec a32723 rrid ab 2633275 goat anti igg h l
A32723 Rrid Ab 2633275 Goat Anti Igg H L, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alexfluor 568 goat anti-rabbit  (Thermo Fisher)
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Thermo Fisher alexfluor 568 goat anti-rabbit
Alexfluor 568 Goat Anti Rabbit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alexa fluor plus 488  (Thermo Fisher)
86
Thermo Fisher alexa fluor plus 488
Expression of marker proteins in regenerated nerves. (A) Immunofluorescence for NF200 (green, Alexa fluor Plus 488), MBP (red, Alexa fluor Plus 594), and S100 (purple, Alexa fluor Plus 647) in transverse sections of the distal nerve stumps in the six groups: Autograft (A1), SE-miR-221/338 (A2), Co-miR-221/338 (A3), miR-338 (A4), miR-221 (A5), and GV369/CV051 (A6) groups at 3 months post-operation. Nuclei were stained with DAPI (blue). The fluorescence expressions of NF200, MBP, and S100 were enhanced in A1 and A2 groups compared with those in other groups. Scale bars: 50 μm. (B–E) Western blot assay was used to detect the protein expression of NF200 (C), S100 (D), and MBP (E) of the distal nerve stumps. B1–6: Autograft (B1), SE-miR-221/338 (B2), Co-miR-221/338 (B3), miR-338 (B4), miR-221 (B5), and GV369/CV051 (B6) groups. NF200, F (5, 53) = 73.81, P < 0.0001; S100, F (5, 53) = 38.14, P < 0.0001; MBP, F (5, 53) = 49.04, P < 0.0001. Data are expressed as the mean ± SD ( n = 9). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance with Tukey’s post hoc tests). Co: Co-expression; DAPI: 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; MBP: myelin basic protein; NF200: neurofilament; SE: sequential expression..
Alexa Fluor Plus 488, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alexa fluor 488/594 goat antimouse  (Thermo Fisher)
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Thermo Fisher alexa fluor 488/594 goat antimouse
Expression of marker proteins in regenerated nerves. (A) Immunofluorescence for NF200 (green, Alexa fluor Plus 488), MBP (red, Alexa fluor Plus 594), and S100 (purple, Alexa fluor Plus 647) in transverse sections of the distal nerve stumps in the six groups: Autograft (A1), SE-miR-221/338 (A2), Co-miR-221/338 (A3), miR-338 (A4), miR-221 (A5), and GV369/CV051 (A6) groups at 3 months post-operation. Nuclei were stained with DAPI (blue). The fluorescence expressions of NF200, MBP, and S100 were enhanced in A1 and A2 groups compared with those in other groups. Scale bars: 50 μm. (B–E) Western blot assay was used to detect the protein expression of NF200 (C), S100 (D), and MBP (E) of the distal nerve stumps. B1–6: Autograft (B1), SE-miR-221/338 (B2), Co-miR-221/338 (B3), miR-338 (B4), miR-221 (B5), and GV369/CV051 (B6) groups. NF200, F (5, 53) = 73.81, P < 0.0001; S100, F (5, 53) = 38.14, P < 0.0001; MBP, F (5, 53) = 49.04, P < 0.0001. Data are expressed as the mean ± SD ( n = 9). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance with Tukey’s post hoc tests). Co: Co-expression; DAPI: 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; MBP: myelin basic protein; NF200: neurofilament; SE: sequential expression..
Alexa Fluor 488/594 Goat Antimouse, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti-mouse igg (h + l) alexa fluor 488  (Thermo Fisher)
90
Thermo Fisher goat anti-mouse igg (h + l) alexa fluor 488
Expression of marker proteins in regenerated nerves. (A) Immunofluorescence for NF200 (green, Alexa fluor Plus 488), MBP (red, Alexa fluor Plus 594), and S100 (purple, Alexa fluor Plus 647) in transverse sections of the distal nerve stumps in the six groups: Autograft (A1), SE-miR-221/338 (A2), Co-miR-221/338 (A3), miR-338 (A4), miR-221 (A5), and GV369/CV051 (A6) groups at 3 months post-operation. Nuclei were stained with DAPI (blue). The fluorescence expressions of NF200, MBP, and S100 were enhanced in A1 and A2 groups compared with those in other groups. Scale bars: 50 μm. (B–E) Western blot assay was used to detect the protein expression of NF200 (C), S100 (D), and MBP (E) of the distal nerve stumps. B1–6: Autograft (B1), SE-miR-221/338 (B2), Co-miR-221/338 (B3), miR-338 (B4), miR-221 (B5), and GV369/CV051 (B6) groups. NF200, F (5, 53) = 73.81, P < 0.0001; S100, F (5, 53) = 38.14, P < 0.0001; MBP, F (5, 53) = 49.04, P < 0.0001. Data are expressed as the mean ± SD ( n = 9). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance with Tukey’s post hoc tests). Co: Co-expression; DAPI: 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; MBP: myelin basic protein; NF200: neurofilament; SE: sequential expression..
Goat Anti Mouse Igg (H + L) Alexa Fluor 488, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alexa fluor 488 antibody  (Fisher Scientific)
90
Fisher Scientific alexa fluor 488 antibody
Expression of marker proteins in regenerated nerves. (A) Immunofluorescence for NF200 (green, Alexa fluor Plus 488), MBP (red, Alexa fluor Plus 594), and S100 (purple, Alexa fluor Plus 647) in transverse sections of the distal nerve stumps in the six groups: Autograft (A1), SE-miR-221/338 (A2), Co-miR-221/338 (A3), miR-338 (A4), miR-221 (A5), and GV369/CV051 (A6) groups at 3 months post-operation. Nuclei were stained with DAPI (blue). The fluorescence expressions of NF200, MBP, and S100 were enhanced in A1 and A2 groups compared with those in other groups. Scale bars: 50 μm. (B–E) Western blot assay was used to detect the protein expression of NF200 (C), S100 (D), and MBP (E) of the distal nerve stumps. B1–6: Autograft (B1), SE-miR-221/338 (B2), Co-miR-221/338 (B3), miR-338 (B4), miR-221 (B5), and GV369/CV051 (B6) groups. NF200, F (5, 53) = 73.81, P < 0.0001; S100, F (5, 53) = 38.14, P < 0.0001; MBP, F (5, 53) = 49.04, P < 0.0001. Data are expressed as the mean ± SD ( n = 9). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance with Tukey’s post hoc tests). Co: Co-expression; DAPI: 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; MBP: myelin basic protein; NF200: neurofilament; SE: sequential expression..
Alexa Fluor 488 Antibody, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti mouse alexa fluor 488  (Thermo Fisher)
86
Thermo Fisher goat anti mouse alexa fluor 488
Expression of marker proteins in regenerated nerves. (A) Immunofluorescence for NF200 (green, Alexa fluor Plus 488), MBP (red, Alexa fluor Plus 594), and S100 (purple, Alexa fluor Plus 647) in transverse sections of the distal nerve stumps in the six groups: Autograft (A1), SE-miR-221/338 (A2), Co-miR-221/338 (A3), miR-338 (A4), miR-221 (A5), and GV369/CV051 (A6) groups at 3 months post-operation. Nuclei were stained with DAPI (blue). The fluorescence expressions of NF200, MBP, and S100 were enhanced in A1 and A2 groups compared with those in other groups. Scale bars: 50 μm. (B–E) Western blot assay was used to detect the protein expression of NF200 (C), S100 (D), and MBP (E) of the distal nerve stumps. B1–6: Autograft (B1), SE-miR-221/338 (B2), Co-miR-221/338 (B3), miR-338 (B4), miR-221 (B5), and GV369/CV051 (B6) groups. NF200, F (5, 53) = 73.81, P < 0.0001; S100, F (5, 53) = 38.14, P < 0.0001; MBP, F (5, 53) = 49.04, P < 0.0001. Data are expressed as the mean ± SD ( n = 9). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance with Tukey’s post hoc tests). Co: Co-expression; DAPI: 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; MBP: myelin basic protein; NF200: neurofilament; SE: sequential expression..
Goat Anti Mouse Alexa Fluor 488, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s1250 gefitinib selleck chemical  (Selleck Chemicals)
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Selleck Chemicals s1250 gefitinib selleck chemical
Expression of marker proteins in regenerated nerves. (A) Immunofluorescence for NF200 (green, Alexa fluor Plus 488), MBP (red, Alexa fluor Plus 594), and S100 (purple, Alexa fluor Plus 647) in transverse sections of the distal nerve stumps in the six groups: Autograft (A1), SE-miR-221/338 (A2), Co-miR-221/338 (A3), miR-338 (A4), miR-221 (A5), and GV369/CV051 (A6) groups at 3 months post-operation. Nuclei were stained with DAPI (blue). The fluorescence expressions of NF200, MBP, and S100 were enhanced in A1 and A2 groups compared with those in other groups. Scale bars: 50 μm. (B–E) Western blot assay was used to detect the protein expression of NF200 (C), S100 (D), and MBP (E) of the distal nerve stumps. B1–6: Autograft (B1), SE-miR-221/338 (B2), Co-miR-221/338 (B3), miR-338 (B4), miR-221 (B5), and GV369/CV051 (B6) groups. NF200, F (5, 53) = 73.81, P < 0.0001; S100, F (5, 53) = 38.14, P < 0.0001; MBP, F (5, 53) = 49.04, P < 0.0001. Data are expressed as the mean ± SD ( n = 9). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance with Tukey’s post hoc tests). Co: Co-expression; DAPI: 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; MBP: myelin basic protein; NF200: neurofilament; SE: sequential expression..
S1250 Gefitinib Selleck Chemical, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phg0314 enzalutamide selleck chemical  (Selleck Chemicals)
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Selleck Chemicals phg0314 enzalutamide selleck chemical
Expression of marker proteins in regenerated nerves. (A) Immunofluorescence for NF200 (green, Alexa fluor Plus 488), MBP (red, Alexa fluor Plus 594), and S100 (purple, Alexa fluor Plus 647) in transverse sections of the distal nerve stumps in the six groups: Autograft (A1), SE-miR-221/338 (A2), Co-miR-221/338 (A3), miR-338 (A4), miR-221 (A5), and GV369/CV051 (A6) groups at 3 months post-operation. Nuclei were stained with DAPI (blue). The fluorescence expressions of NF200, MBP, and S100 were enhanced in A1 and A2 groups compared with those in other groups. Scale bars: 50 μm. (B–E) Western blot assay was used to detect the protein expression of NF200 (C), S100 (D), and MBP (E) of the distal nerve stumps. B1–6: Autograft (B1), SE-miR-221/338 (B2), Co-miR-221/338 (B3), miR-338 (B4), miR-221 (B5), and GV369/CV051 (B6) groups. NF200, F (5, 53) = 73.81, P < 0.0001; S100, F (5, 53) = 38.14, P < 0.0001; MBP, F (5, 53) = 49.04, P < 0.0001. Data are expressed as the mean ± SD ( n = 9). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance with Tukey’s post hoc tests). Co: Co-expression; DAPI: 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; MBP: myelin basic protein; NF200: neurofilament; SE: sequential expression..
Phg0314 Enzalutamide Selleck Chemical, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gm6001 selleck chemical  (Selleck Chemicals)
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Expression of marker proteins in regenerated nerves. (A) Immunofluorescence for NF200 (green, Alexa fluor Plus 488), MBP (red, Alexa fluor Plus 594), and S100 (purple, Alexa fluor Plus 647) in transverse sections of the distal nerve stumps in the six groups: Autograft (A1), SE-miR-221/338 (A2), Co-miR-221/338 (A3), miR-338 (A4), miR-221 (A5), and GV369/CV051 (A6) groups at 3 months post-operation. Nuclei were stained with DAPI (blue). The fluorescence expressions of NF200, MBP, and S100 were enhanced in A1 and A2 groups compared with those in other groups. Scale bars: 50 μm. (B–E) Western blot assay was used to detect the protein expression of NF200 (C), S100 (D), and MBP (E) of the distal nerve stumps. B1–6: Autograft (B1), SE-miR-221/338 (B2), Co-miR-221/338 (B3), miR-338 (B4), miR-221 (B5), and GV369/CV051 (B6) groups. NF200, F (5, 53) = 73.81, P < 0.0001; S100, F (5, 53) = 38.14, P < 0.0001; MBP, F (5, 53) = 49.04, P < 0.0001. Data are expressed as the mean ± SD ( n = 9). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance with Tukey’s post hoc tests). Co: Co-expression; DAPI: 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; MBP: myelin basic protein; NF200: neurofilament; SE: sequential expression..
Gm6001 Selleck Chemical, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s 7979 src inhibitor 1 selleck chemical  (Selleck Chemicals)
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Expression of marker proteins in regenerated nerves. (A) Immunofluorescence for NF200 (green, Alexa fluor Plus 488), MBP (red, Alexa fluor Plus 594), and S100 (purple, Alexa fluor Plus 647) in transverse sections of the distal nerve stumps in the six groups: Autograft (A1), SE-miR-221/338 (A2), Co-miR-221/338 (A3), miR-338 (A4), miR-221 (A5), and GV369/CV051 (A6) groups at 3 months post-operation. Nuclei were stained with DAPI (blue). The fluorescence expressions of NF200, MBP, and S100 were enhanced in A1 and A2 groups compared with those in other groups. Scale bars: 50 μm. (B–E) Western blot assay was used to detect the protein expression of NF200 (C), S100 (D), and MBP (E) of the distal nerve stumps. B1–6: Autograft (B1), SE-miR-221/338 (B2), Co-miR-221/338 (B3), miR-338 (B4), miR-221 (B5), and GV369/CV051 (B6) groups. NF200, F (5, 53) = 73.81, P < 0.0001; S100, F (5, 53) = 38.14, P < 0.0001; MBP, F (5, 53) = 49.04, P < 0.0001. Data are expressed as the mean ± SD ( n = 9). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance with Tukey’s post hoc tests). Co: Co-expression; DAPI: 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; MBP: myelin basic protein; NF200: neurofilament; SE: sequential expression..
S 7979 Src Inhibitor 1 Selleck Chemical, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Expression of marker proteins in regenerated nerves. (A) Immunofluorescence for NF200 (green, Alexa fluor Plus 488), MBP (red, Alexa fluor Plus 594), and S100 (purple, Alexa fluor Plus 647) in transverse sections of the distal nerve stumps in the six groups: Autograft (A1), SE-miR-221/338 (A2), Co-miR-221/338 (A3), miR-338 (A4), miR-221 (A5), and GV369/CV051 (A6) groups at 3 months post-operation. Nuclei were stained with DAPI (blue). The fluorescence expressions of NF200, MBP, and S100 were enhanced in A1 and A2 groups compared with those in other groups. Scale bars: 50 μm. (B–E) Western blot assay was used to detect the protein expression of NF200 (C), S100 (D), and MBP (E) of the distal nerve stumps. B1–6: Autograft (B1), SE-miR-221/338 (B2), Co-miR-221/338 (B3), miR-338 (B4), miR-221 (B5), and GV369/CV051 (B6) groups. NF200, F (5, 53) = 73.81, P < 0.0001; S100, F (5, 53) = 38.14, P < 0.0001; MBP, F (5, 53) = 49.04, P < 0.0001. Data are expressed as the mean ± SD ( n = 9). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance with Tukey’s post hoc tests). Co: Co-expression; DAPI: 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; MBP: myelin basic protein; NF200: neurofilament; SE: sequential expression..

Journal: Neural Regeneration Research

Article Title: Sequential expression of miR-221-3p and miR-338-3p in Schwann cells as a therapeutic strategy to promote nerve regeneration and functional recovery

doi: 10.4103/1673-5374.350214

Figure Lengend Snippet: Expression of marker proteins in regenerated nerves. (A) Immunofluorescence for NF200 (green, Alexa fluor Plus 488), MBP (red, Alexa fluor Plus 594), and S100 (purple, Alexa fluor Plus 647) in transverse sections of the distal nerve stumps in the six groups: Autograft (A1), SE-miR-221/338 (A2), Co-miR-221/338 (A3), miR-338 (A4), miR-221 (A5), and GV369/CV051 (A6) groups at 3 months post-operation. Nuclei were stained with DAPI (blue). The fluorescence expressions of NF200, MBP, and S100 were enhanced in A1 and A2 groups compared with those in other groups. Scale bars: 50 μm. (B–E) Western blot assay was used to detect the protein expression of NF200 (C), S100 (D), and MBP (E) of the distal nerve stumps. B1–6: Autograft (B1), SE-miR-221/338 (B2), Co-miR-221/338 (B3), miR-338 (B4), miR-221 (B5), and GV369/CV051 (B6) groups. NF200, F (5, 53) = 73.81, P < 0.0001; S100, F (5, 53) = 38.14, P < 0.0001; MBP, F (5, 53) = 49.04, P < 0.0001. Data are expressed as the mean ± SD ( n = 9). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance with Tukey’s post hoc tests). Co: Co-expression; DAPI: 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; MBP: myelin basic protein; NF200: neurofilament; SE: sequential expression..

Article Snippet: Samples were then incubated with goat anti-chicken secondary antibody (1:500) conjugated with Alexa Fluor® Plus 594 (Thermo Fisher Scientific, Cat# A32759, RRID: AB_2762829), goat anti-mouse secondary antibody (1:500) conjugated with Alexa Fluor® Plus 488 (Thermo Fisher Scientific, Cat# A32723, RRID: AB_2633275), and goat anti-rabbit secondary antibody (1:500) conjugated with Alexa Fluor® Plus 647 (Thermo Fisher Scientific Cat# A32733, RRID: AB_2633282).

Techniques: Expressing, Marker, Immunofluorescence, Staining, Fluorescence, Western Blot